Histone H2A (1-129 aa), Xenopus recombinant | 7668

H2A is a core component of nucleosome. A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around an octamer of histones. The octamer is composed of two of each histone H2A, H2B, H3, and H4. DNA accessibility is regulated via a complex set of post-translational modifications of these histones, also called histone code, and nucleosome remodeling. Histones H2A and H2B form a dimer. Histones H3 and H4 form a tetramer. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core. Histone H3 undergoes many modifications which include acetylation, phosphorylation, ubiquitylation, sumoylation, and biotinylation that are important for regulation of gene transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

7668 | Histone H2A 1-129 aa Xenopus recombinant DataSheet

Biomolecule/Target: Histone H2A

Synonyms: Histone H2A (1-129 aa), Xenopus recombinant

Alternates names: Insulin-like Growth Factor-Binding Protein5

Taglines: Core component of nucleosome

NCBI Gene ID #: 3488

NCBI Gene Symbol: IBP5

Gene Source: N/A

Accession #: P24593

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is <0.3 mg/ml, in the presence of 15 ng/ml of human IGF-II.

Results: <0.3 mg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28. 6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder. Recommended buffer is 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride, and 20% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A