Additional Information
Size: |
100 µl |
Target: |
Histone H2A.X (pSer139) |
Conjugate: |
APC/Cy7 |
Research Area: |
Cell Signaling | Epigenetics and Nuclear Signaling | Histones | DNA/RNA | DNA Damage and Repair | DNA Damage Recognition |
Alternative Name: |
H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E). |
Immunogen Species: |
Human |
Applications: |
WB, ICC/IF |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
WB (1:1000); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Cellular Localization: |
Nucleus, Chromosome |
Tissue Specificity: |
N/A |
Accession Number: |
NP_002096.1 |
Gene ID: |
3014 |
Swiss-Prot: |
P16104 |
Field of Use: |
For in vitro research use only. |