Additional Information
| Size: | 100 µl | 
| Target: | Histone H2A.X (pSer139) | 
| Conjugate: | ATTO 565 | 
| Research Area: | Cell Signaling | Epigenetics and Nuclear Signaling | Histones | DNA/RNA | DNA Damage and Repair | DNA Damage Recognition | 
| Alternative Name: | H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody | 
| Category: | Antibodies | 
| Product Type: | Polyclonal Antibody | 
| Immunogen: | Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E). | 
| Immunogen Species: | Human | 
| Applications: | WB, ICC/IF | 
| Host: | Rabbit | 
| Isotype: | IgG | 
| Species Reactivity: | Human, Mouse, Rat | 
| Antibody Dilution: | WB (1:1000); optimal dilutions for assays should be determined by the user. | 
| Purification: | Peptide Affinity Purified | 
| Storage Buffer: | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide | 
| Concentration: | N/A | 
| Specificity: | Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139. | 
| Storage: | -20ºC | 
| Shipping: | Blue Ice or 4ºC | 
| Certificate of Analysis: | A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. | 
| Cellular Localization: | Nucleus, Chromosome | 
| Tissue Specificity: | N/A | 
| Accession Number: | NP_002096.1 | 
| Gene ID: | 3014 | 
| Swiss-Prot: | P16104 | 
| Field of Use: | For in vitro research use only. | 
 
             
             
             
             
             
            