Histone H3.3 Rabbit monoclonal Antibody | BS9952M

Histone H3.3 Rabbit monoclonal Antibody | BS9952M | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB ICC/IF IHC CHIP

Application Range: WB: 1:1000-1:2000 IHC/ICC/IF: 1:50-1:200 CHIP: 1:50-1:200

Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of Histone H3.3 and does not cross-react with related proteins.

Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Histone H3.3; H3F3A; H3.3A; H3F3; ORF Names:PP781; H3F3B; H3.3B

Immunogen: Recombinant Antibody.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Protein A affinity purified

Pathway: Tumor-Infiltrating Immune Cell Marker Guide,