Histone H3 (Di-Methyl K14) polyclonal Antibody | BS7524

Histone H3 (Di-Methyl K14) polyclonal Antibody | BS7524 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat,Other (Wide Range)

Application: WB,IHC,IF

Application Range: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Histone H3 (Di-Methyl K14) polyclonal Antibody detects endogenous levels of histone H3 only when Di-methylated at Lys14.

Molecular Weight: ~15 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Histone H3K14me2

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, di methylated at K14.

Conjugate: Unconjugated

Modification: Methylated

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: