Histone H4 (Di-Methyl K20) polyclonal Antibody | BS8450

Histone H4 (Di-Methyl K20) polyclonal Antibody | BS8450 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat,Other (Wide Range)

Application: WB,IHC,IF

Application Range: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Histone H4 (Di-Methyl K20) polyclonal Antibody detects endogenous levels of histone H4 only when Di-Methyled at Lys20.

Molecular Weight: ~ 11 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: H4K20me2

Immunogen: Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H4, Di-methylated at K20.

Conjugate: Unconjugated

Modification: Methylated

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Histone Lysine Methylation Pathway,Examples of Crosstalk Between Post-translational Modifications,DNA Methylation,