LBD
HLA-DRB1 Antibody (Center) [APR04702G]
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- LBD-APR04702G
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Description
HLA-DRB1 Antibody (Center) [APR04702G] | Gentaur UK, US & Europe Distribution
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This HLA-DRB1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 122-154 amino acids from the Central region of human HLA-DRB1.
Cellular Localisation: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Autolysosome membrane Note=The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation (PubMed:18305173) . Component of immunological synapses at the interface between T cell and APC (PubMed:29884618) .
Molecular Weight: 29966
Clone: Polyclonal
Gene Name: HLA-DRB1 (HGNC:4948)
Gene ID: 3123
Function: A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA- DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:29884618, PubMed:22327072, PubMed:27591323, PubMed:8642306, PubMed:15265931, PubMed:31495665, PubMed:16148104) . Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed:8145819) . In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor- resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed:31495665) . Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed:17182262, PubMed:23783831) . The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed:25413013) . The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed:8145819) .
Summary: Tissue Location: Expressed in professional APCs: monocyte/macrophages, dendritic cells and B cells (at protein level) (PubMed:31495665, PubMed:23783831, PubMed:19830726) . Expressed in thymic epithelial cells (at protein level) (PubMed:23783831)
Form: N/A
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: HLA class II histocompatibility antigen, DRB1-15 beta chain, DW22/DR22, MHC class II antigen DRB1*15, HLA-DRB1, HLA-DRB2