HMGA1 Antibody | 45-737

HMGA1 Antibody | 45-737 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: C-LASKQEKDGTEK

Research Area: Cancer

Tested Application: E, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b ( (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1) . The 50kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4ug/ml.

Specificiy: This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1) .

Positive Control 1: Cat. No. 1309 - Human Placenta Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: HMGA1, high mobility group AT-hook 1, HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854, OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A, OTTHUMP00000016223, OTTHUMP00000016224

User Note: N/A

BACKGROUND: N/A