HMGB1 Antibody | 7715

HMGB1 Antibody | 7715 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (100%) , Pig: (100%)

Immunogen: Anti-HMGB1 antibody (7715) was raised against a peptide corresponding to 19 amino acids near the center of human HMGB1.
The immunogen is located within 70-120 amino acids of HMGB1.

Research Area: Innate Immunity

Tested Application: E, WB, IHC-P, IF

Application: WB: 1 μg/mL; IHC: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: HMGB1 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 25 kDa
Observed: 25 kDa

Validation:

KO Validation (Figure 1) : Anti-HMGB1 antibodies (7715) specificity was further verified by HMGB1 specific knockout cell lysate. HMGB1 signal was detected in wild type cells, but not in the HMGB1 knockout cells.

Isoform: Human HMGB1 has only 1 isoform (215aa, 25kD) . Mouse HMGB1 has only 1 isoform (215aa, 25kD) . Rat HMGB1 has only 1 isoform (215aa, 25kD) . This antibody detects HMGB1 of human, mouse and rat.

Purification: HMGB1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: HMGB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1, 2) . However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS) -induced endotoxemia and sepsis (3) . HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4) .