Homocysteine Lyase Activity Assay Kit (Fluorometric) - K2026

K2026 | Homocysteine Lyase Activity Assay Kit (Fluorometric) DataSheet

Homocysteine α,γ-Lyase (HCYase) is a pyridoxal 5'-phosphate dependent enzyme that catalyzes the breakdown of homocysteine to hydrogen sulfide, ammonia, and α-ketobutyrate. HCYase is present in high activity in the anaerobic protozoan parasite Trichomonas vaginalis but is absent from mammals. The Trichomonas HCYase is highly selective for homocysteine as compared to cysteine. Additionally, it has broad pH stability (pH 6-8) and is thermally stable up to 50°C. This makes rHCYase a promising candidate for therapeutic applications including the diagnosis of hyperhomocystenemia, which has been demonstrated to be a major risk factor in cardiovascular diseases. ’s Homocysteine Lyase Activity Assay Kit is a simple, quick and sensitive method to assay HCYase activity in bacterial samples. The assay relies on the HCYase-catalyzed release of hydrogen sulfide (H2S), which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm.

Alternate Name : Homocysteine Lyase Activity Assay Kit (Fluorometric)

Tag Line : To assay HCYase activity in bacterial samples

Summary : ’s Homocysteine Lyase Activity Assay Kit is a simple, quick and sensitive method to assay HCYase activity in bacterial samples. The assay relies on the HCYase-catalyzed release of hydrogen sulfide (H2S), which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm.

Detection Method : Fluorescence (Ex/Em = 368/460 nm)

Sample Type : Bacterial cell lysate

Species Reactivity : Bacterial samples

Applications : Determining the activity of wild type or recombinant Homocysteine α,γ-Lyase

Features and Benefits : Sensitive method to assay HCYase activity in bacterial samples, Quick, Simple