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Homocysteine ,-Lyase (rHCYase), Active, Recombinant | P1117

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SKU:
26-P1117-GEN
Availability:
Usually shipped in 5 working days
NULL756.00

Description

Recombinant Homocysteine ,-lyase is a pyridoxal-5’-phosphate dependent enzyme. It is a mutant of homocysteinase from Trichomonas vaginalis encoded by mgl1 gene, containing three point mutations, such as; Phe47Leu, Asp172Glu, Ser308Tyr. Its native molecular mass is 170 kD, with four seemingly identical subunits, each with molecular mass of 43-45 kD. The enzyme can metabolize homocysteine into -keto butyrate, hydrogen sulfide and ammonia.

P1117 | Homocysteine -Lyase rHCYase Active Recombinant DataSheet

Biomolecule/Target: mgl1

Synonyms: Homocysteine ,-Lyase (rHCYase)

Alternates names: ,-Homocysteinase, Methionine gamma-lyase, mgl1

Taglines: Enzyme that can metabolize homocysteine into -keto butyrate, hydrogen sulfide and ammonia.

NCBI Gene ID #: 2153

NCBI Gene Symbol: F5

Gene Source: Trichomonas vaginalis

Accession #: P12259

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate

Biological activity: Specific activity is 5 mU/mg in which one Unit enzyme converts 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate

Results: 5 mU/mg

Binding Capacity: N/A

Unit Definition: One Unit enzyme converts 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate

Molecular Weight: 43 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized form proprietary buffer

Reconstitution Instructions: Reconstitute in 40 mM Sodium phosphate buffer, pH 8.1.

Amino acid sequence: N/A

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Additional Information

Storage:
-20°C
Shipping:
Gel Pack
Shelf Life:
12 months
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