HOPX Antibody | 8143

HOPX Antibody | 8143 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (88%) , Mouse: (88%) , Bovine: (88%) , Pig: (81%) , Chicken: (81%)

Immunogen: HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX.
The immunogen is located within the first 50 amino acids of HOPX.

Research Area: Cancer, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 12 kDa
Observed: 18 kDa

Validation: N/A

Isoform: N/A

Purification: HOPX antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: HOPX antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: HOPX antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: HOP homeobox, homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1) . It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF) -dependent cardiac-specific gene expression and cardiac development (1) . HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2, 3) . HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4) .