Horse IgM (Immunoglobulin M) ELISA Kit | ELK8959

Horse IgM (Immunoglobulin M) ELISA Kit | ELK8959 | Gentaur UK, US & Europe Distribution

Alternative Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Species: Horse

Assay Type: Sandwich

Sensitivity: 0.71 ug/mL

Standard: 100 ug/mL

Detection Range: 1.57-100 ug/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin M (IgM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin M (IgM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin M (IgM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin M (IgM) in the samples is then determined by comparing the OD of the samples to the standard curve.