HPRT1, human recombinant | P1092

Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1092 | HPRT1 human recombinant DataSheet

Biomolecule/Target: HPRT1

Synonyms: Hypoxanthine-guanine phosphoribosyltransferase, GPRT, HGPRTase, HPRT

Alternates names: Inhibin beta-1, FRP, FSH-releasing protein

Taglines: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate.

NCBI Gene ID #: 3624

NCBI Gene Symbol: INHBA

Gene Source: Human

Accession #: P08476

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.

Biological activity: The activity is determined by the ability to induce cytoxicity of MPC-11 cells and is typically less than 2 ng/mL.

Results: < 2 ng/mL

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.

Molecular Weight: 26.2 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions.

Amino acid sequence: MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS