Description
hRIP3 Antibody | 8963 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Anti-hRIP3 antibody (8963) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human hRIP3.
The immunogen is located within the last 50 amino acids of hRIP3.
Research Area: Apoptosis
Tested Application: E, IF, IHC-P, WB
Application: WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL.
Antibody validated: Western Blot, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: Several isoforms of hRIP3 are known to exist.
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 1224 - Daudi Cell Lysate
Positive Control 3: Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 57kD
Observed: 57 kD
Validation: N/A
Isoform: Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD) , isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD) . Mouse RIP3 has one isoform (486aa, 53kD) and Rat RIP3 also has one isoform (478aa, 52kD) . 8963 can detect only human isoform 1 and cannot detect other human isoforms, mouse and rat isoforms.
Purification: hRIP3 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: hRIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: hRIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: hRIP3 Antibody: Rip3, AW107945, 2610528K09Rik, RIP-like protein kinase 3, RIP-3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: hRIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.