Additional Information
Size:  | 
                                                    100 µg  | 
                                                
Other Name:  | 
                                                    HSF1 Antibody: APC  | 
                                                
Target:  | 
                                                    HSF1  | 
                                                
Conjugate:  | 
                                                    APC  | 
                                                
Research Area:  | 
                                                    Cancer, Heat Shock, Cell Signaling, Epigenetics and Nuclear Signaling, Cardiovascular System, Heart  | 
                                                
Alternative Name(s):  | 
                                                    HSTF1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody  | 
                                                
Product Type:  | 
                                                    Monoclonal Antibody  | 
                                                
Clone Number:  | 
                                                    10H8  | 
                                                
Immunogen:  | 
                                                    Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395  | 
                                                
Immunogen Species:  | 
                                                    Mouse  | 
                                                
Applications:  | 
                                                    WB, IHC, ICC/IF, IP, ELISA, GS  | 
                                                
Host:  | 
                                                    Rat  | 
                                                
Isotype:  | 
                                                    IgG1  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse, Rat, Bovine, Guinea Pig (Cavia porcellus), Hamster, Monkey, Rabbit  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:1000), IHC (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Protein G Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS pH7.4, 50% glycerol, 0.09% sodium azide  | 
                                                
Concentration:  | 
                                                    1 mg/ml  | 
                                                
Specificity:  | 
                                                    Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    1 µg/ml of SMC-118 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody  | 
                                                
Cellular Localization:  | 
                                                    Cytoplasm | Nucleus  | 
                                                
Accession Number:  | 
                                                    NP_032322.1  | 
                                                
Gene ID:  | 
                                                    15499  | 
                                                
Swiss-Prot:  | 
                                                    P38532  | 
                                                
Field of Use:  | 
                                                    Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.  |