HSP18, 5 | class IV heat shock protein | AS11 1628

HSP18, 5 | class IV heat shock protein | AS11 1628 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinat Arabidopsis thaliana HSP18.5, expressed in E.coli, UniProt: O64564, TAIR: AT2G19310

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 18, 5 kDa

Confirmed Reactivity: Arabidopsis thaliana, Hordeum vulgare

Predicted Reactivity: Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea mays Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

Background: HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G19310

Category: Heat shock

Research Area: Plant Biology