HSP90α Inhibitor Screening Kit (Fluorometric) | K2106-10

HSP90α Inhibitor Screening Kit (Fluorometric) | K2106-100

Description: Heat shock protein (HSP) 90 is an ATP-dependent molecular chaperone that assists other proteins to fold properly, stabilizes proteins against heat stress, and assists in protein degradation. It has two isoforms (HSP90α and HSP90β) and both are abundantly expressed in the cytoplasm. Heat stress can cause proteins to denature thereby resulting in the loss of protein function. However, HSP90 can bind to the proteins during heat stress and protect them from misfolding and aggregation. Since HSP90 stabilizes various proteins including cancer-related proteins, cancer-associated protein kinases, transcription factors etc. that are required for the survival of cancer cells, HSP90 is considered as a potential target for cancer therapeutics. HSP90 inhibitors can target either the N-terminal ATP binding site or the C-terminal site of HSP90. Additionally, a number of HSP90 inhibitors are in clinical trials. ’s0α Inhibitor Screening Kit is a 96-well fluorometric assay that can be used to screen and characterize the potential inhibitors of HSP90α. The assay is based on measuring the HSP90-dependent refolding of thermally denatured Lactate Dehydrogenase (LDH) in the presence and absence of potential HSP90α inhibitors. In the presence of potential HSP90α inhibitors, refolding of thermally denatured LDH will be arrested resulting in reduced LDH activity, which is measured by fluorescence at Ex/Em = 535/587 nm. Geldanamycin is included in the kit as a positive control. The assay is simple, rapid and provides a reliable test for screening HSP90α inhibitors.

Alternate Name: Heat shock protein Inhibitor Screening Kit Heat shock protein Inhibitor Screening Kit (Fluorometric) HSP90α Inhibitor Screening Kit Heat shock proteinα Inhibitor Screening Kit

Tag Line: fOR Screening or characterizing HSP90α inhibitors

Summary: ’s HSP90α Inhibitor Screening Kit is a 96-well flussay that can be used to screen and characterize the potential inhibitors of HSP90α.

Detection Method: Fluorescence (Ex/Em = 535/587 nm)

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Applications: For screening or characterizing HSP90α inhibitors

Features & Benefits: The assay is simple, rapid and provides a reliable test for screening HSP90α inhibito