HSP90B1 Antibody | 45-071

HSP90B1 Antibody | 45-071 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow, Zebrafish

Immunogen: The immunogen for this antibody is: C-KEGVKFDESEKTKE

Research Area: Neuroscience

Tested Application: E, WB, IHC, Flow, IF

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5kDa according to Human NP_003290.1 and Mouse NP_035761.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5µg/ml. Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TRA1, GRP94, tumor rejection antigen (gp96) 1, HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96) , endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1

User Note: N/A

BACKGROUND: N/A