HSV I /HSV II / T. pallidum RT-PCR | B87-FRT

HSV I /HSV II / T. pallidum RT-PCR (CE) | B87-FRT is available for delivery

Multiplex Real Time PCR kit for qualitative detection of HSV I, HSV II and Treponema pallidum

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

HSV I /HSV II / T. pallidum RT-PCR B87-FRT DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gonorrhea, herpes, HIV, HPV, syphilis, gardnerella and trichomoniasis.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

HSV I/HSV II/T. pallidum Real-TM PCR kit is an in vitro nucleic acid amplification test for multiplex detection of HSV I, HSV II and Treponema pallidum DNA in clinical materials (urogenital, rectal and pharyngeal swabs; conjunctival discharge; prostate gland secretion; urine samples) by using real-time hybridization-fluorescence detection.

PRINCIPLE OF ASSAY

HSV I, HSV II and Treponema pallidum detection by the multiplex polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific HSV I, HSV II and Treponema pallidum primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes which bind specifically to the amplified product during thermocycling. The real-time monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run. HSV I/HSV II/T. pallidum Real-TM PCR kit is a qualitative test that contains the Internal Control (IC). It must be used in the extraction procedure in order to control the extraction process of each individual sample and to identify possible reaction inhibition.

HSV I/HSV II/T. pallidum Real-TM PCR kit uses “hot-start”, which greatly reduces frequency of nonspecifically primed reactions. “Hot-start” is guaranteed by chemically modified polymerase (TaqF), which is activated by heating at 95 ºC for 15 min.