Human 17-ketosteroids (17-KS) ELISA Kit | AE22896HU

Human 17-ketosteroids (17-KS) ELISA Kit | AE22896HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: 17-KS

Alternative Name: N/A

Application: ELISA

Range: 3.125-200 ng/mL

Sensitivity: 1.25 ng/mL

Intra-Assay: ≤7.1%

Inter-Assay: ≤8.5%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate 17-KS in samples. An antibody specific for 17-KS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17-KS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 17-KS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17-KS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: A 17-ketosteroid is a steroid that has a ketone (C=O) functional group at the C17 position (in the upper right corner of most diagrams.) These compound are products of metabolization of testosterone in most tissues such as liver.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .