Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (UGT8) ELISA Kit | KTE62307

Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (UGT8) ELISA Kit | KTE62307 | Gentaur UK, US & Europe Distribution

Application: This Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (UGT8) ELISA Kit employs a two-site sandwich ELISA to quantitate UGT8 in samples. An antibody specific for UGT8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUGT8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UGT8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UGT8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase microplate
• Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase standard
• Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (UGT8) ELISA Kit has high sensitivity and excellent specificity for detection of Human UGT8. No significant cross-reactivity or interference between Human UGT8 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT) . The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.The same part of the predicted amino acid sequence that binds the ceramide moiety binds aglycone in isozymes of the UGT subfamilies. Only subtle changes of the rather conserved C-terminal domain appeared to be sufficient to confer the specificity of the binding site for the activated sugar donor molecule UDP-galactose.

Alternative Names: UGT8; CGT; UGT4; 2-hydroxyacylsphingosine 1-beta-galactosyltransferase; UDP-galactose ceramide galactosyltransferase; UDP-galactose-ceramide galactosyltransferase 8; cerebroside synthase; uridine diphosphate

Search name: UGT8; CGT; UGT4; 2-hydroxyacylsphingosine 1-beta-galactosyltransferase; UDP-galactose ceramide galactosyltransferase; UDP-galactose-ceramide galactosyltransferase 8; cerebroside synthase; uridine diphosphate

Tag: UGT8