Human 4-Hydroxynonenal (HNE) ELISA Kit | ELK8372

Human 4-Hydroxynonenal (HNE) ELISA Kit | ELK8372 | Gentaur UK, US & Europe Distribution

Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 19.1 pg/mL

Standard: 2500 pg/mL

Detection Range: 39.07-2500 pg/mL

Sample Type: Serum, plasma, tissue homogenates

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HNE (4-Hydroxynonenal ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HNE (4-Hydroxynonenal ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNE (4-Hydroxynonenal ) in the samples is then determined by comparing the OD of the samples to the standard curve.