Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit | KTE61243

Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit | KTE61243 | Gentaur UK, US & Europe Distribution

Application: This Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKM in samples. An antibody specific for PFKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human 6-phosphofructokinase, muscle type microplate
• Human 6-phosphofructokinase, muscle type standard
• Human 6-phosphofructokinase, muscle type detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human 6-phosphofructokinase, muscle type (PFKM) ELISA Kit has high sensitivity and excellent specificity for detection of Human PFKM. No significant cross-reactivity or interference between Human PFKM and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Phosphofructokinase-1 (PFK-1) is the most important regulatory enzyme (EC 2.7.1.11) of glycolysis. It is an allosteric enzyme made of 4 subunits and controlled by several activators and inhibitors. PFK-1 catalyzes one of the important "committed" steps of glycolysis, the conversion of fructose 6-phosphate and ATP to fructose 1, 6-bisphosphate and ADP. PFK1 is allosterically inhibited by ATP and citrate (from the citric acid cycle) and its product. It is also inhibited by low pH to prevent the accumulation of hydrogen ions in muscle. The enzyme has two sites with different affinities for ATP which is both a substrate and an inhibitor.PFK1 is allosterically activated by a high concentration of AMP, but the most potent activator is fructose 2, 6-bisphosphate, which is also produced from fructose-6-phosphate by PFK2.

Alternative Names: PFKM; GSD7; MGC8699; PFK-1; PFK1; PFKA; PFKX; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase; polypeptide X; phosphohexokinase

Search name: PFKM; GSD7; MGC8699; PFK-1; PFK1; PFKA; PFKX; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase; polypeptide X; phosphohexokinase

Tag: PFKM