Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA kit | ELK8402

Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA kit | ELK8402 | Gentaur UK, US & Europe Distribution

Alternative Names: 8-isoprostane; 8-iso-PGF2α

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 8.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-iso-PGF2a (8-iso prostaglandin F2α ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-iso-PGF2a (8-iso prostaglandin F2α ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-iso-PGF2a (8-iso prostaglandin F2α ) in the samples is then determined by comparing the OD of the samples to the standard curve.