Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit | KTE60916

Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit | KTE60916 | Gentaur UK, US & Europe Distribution

Application: This Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM8 in samples. An antibody specific for ADAM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human A Disintegrin And Metalloprotease 8 microplate
• Human A Disintegrin And Metalloprotease 8 standard
• Human A Disintegrin And Metalloprotease 8 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM8. No significant cross-reactivity or interference between Human ADAM8 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ADAM8 is expressed in macrophages, granulocytes, monocytes and B cells. It is an osteoclast stimulating factor. ADAM8 may play a role in neuron-glia interactions during neurodegeneration. It may also mediate extravasation of leukocytes either by degrading the vascular basement membrane or by liberating active molecules from their precursors harbored in the cell surface of leukocytes and endothelial cells. Human ADAM8 precursor consists of 824 amino acid (aa) residues consisting of a 16 aa signal peptide, a 637 aa ectodomain including pro-, metalloprotease, disintegrin-like and cysteine-rich regions, a 25 aa transmembrane domain, and a 146 aa cytoplasmic domain) . ADAM8 naturally occurs as both a membrane and a soluble form.

Alternative Names: ADAM8; CD156; MGC134985; MS2; a disintegrin and metalloproteinase domain 8; cell surface antigen MS2; disintegrin and metalloproteinase domain-containing protein 8; human leukocyte differentiation antigen

Search name: ADAM8; CD156; MGC134985; MS2; a disintegrin and metalloproteinase domain 8; cell surface antigen MS2; disintegrin and metalloproteinase domain-containing protein 8; human leukocyte differentiation antigen

Tag: ADAM8