Human A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit | KTE62225

Human A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit | KTE62225 | Gentaur UK, US & Europe Distribution

Application: This Human A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit employs a two-site sandwich ELISA to quantitate MUTYH in samples. An antibody specific for MUTYH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUTYH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUTYH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUTYH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human A/G-specific adenine DNA glycosylase microplate
• Human A/G-specific adenine DNA glycosylase standard
• Human A/G-specific adenine DNA glycosylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUTYH. No significant cross-reactivity or interference between Human MUTYH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MUTYH glycosylase, involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7, 8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in the MUTYH gene cause an autosomal recessive form of familial adenomatous polyposis. Polyps caused by mutated M

Alternative Names: MUTYH; RP4-534D1.2; CYP2C; MGC4416; MYH; A/G-specific adenine DNA glycosylase; OTTHUMP00000009098; OTTHUMP00000009102; mutY homolog

Search name: MUTYH; RP4-534D1.2; CYP2C; MGC4416; MYH; A/G-specific adenine DNA glycosylase; OTTHUMP00000009098; OTTHUMP00000009102; mutY homolog

Tag: MUTYH