855

Human a1M (alpha 1 microglobulin) ELISA Kit | ELK8606

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SKU:
855-ELK8606-GEN
NULL588.00 - NULL704.00

Description

Human a1M (alpha 1 microglobulin) ELISA Kit | ELK8606 | Gentaur UK, US & Europe Distribution

Alternative Names: Alpha-1-Microglobulin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.56 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Kidney biomarker

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to alpha 1 microglobulin (a1M) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to alpha 1 microglobulin (a1M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain alpha 1 microglobulin (a1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of alpha 1 microglobulin (a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.

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