Human ACTN4 (Actinin Alpha 4) ELISA Kit | ELK4564

Human ACTN4 (Actinin Alpha 4) ELISA Kit | ELK4564 | Gentaur UK, US & Europe Distribution

Alternative Names: FSGS; FSGS1; F-actin cross-linking protein; Non-muscle alpha-actinin 4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.32 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 4 (ACTN4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Actinin Alpha 4 (ACTN4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actinin Alpha 4 (ACTN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actinin Alpha 4 (ACTN4) in the samples is then determined by comparing the OD of the samples to the standard curve.