Human ADCYAP1 (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit | ELK4860

Human ADCYAP1 (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit | ELK4860 | Gentaur UK, US & Europe Distribution

Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.55 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.