Human AFM (Afamin) ELISA Kit | ELK3008

Human AFM (Afamin) ELISA Kit | ELK3008 | Gentaur UK, US & Europe Distribution

Alternative Names: ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

Species: Human

Assay Type: Sandwich

Sensitivity: 1.48 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Afamin (AFM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Afamin (AFM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Afamin (AFM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Afamin (AFM) in the samples is then determined by comparing the OD of the samples to the standard curve.