Human aHSP (Alpha-Hemoglobin Stabilizing Protein) ELISA Kit | ELK2360

Human aHSP (Alpha-Hemoglobin Stabilizing Protein) ELISA Kit | ELK2360 | Gentaur UK, US & Europe Distribution

Alternative Names: A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor

Species: Human

Assay Type: Sandwich

Sensitivity: 13.3 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Hemoglobin Stabilizing Protein (aHSP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Hemoglobin Stabilizing Protein (aHSP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Hemoglobin Stabilizing Protein (aHSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Hemoglobin Stabilizing Protein (aHSP) in the samples is then determined by comparing the OD of the samples to the standard curve.