Human ALOX12 (Arachidonate-12-Lipoxygenase) ELISA Kit | ELK4754

Human ALOX12 (Arachidonate-12-Lipoxygenase) ELISA Kit | ELK4754 | Gentaur UK, US & Europe Distribution

Alternative Names: LOX12; 12-LOX; ALOX-12; 12-LO; 12LPG; LOG12; Lipoxygenase 12; 12S-lipoxygenase; Lipoxin synthase 12-LO; Platelet-type lipoxygenase 12

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the OD of the samples to the standard curve.