Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) ELISA Kit | KTE60733

Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) ELISA Kit | KTE60733 | Gentaur UK, US & Europe Distribution

Application: This Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) ELISA Kit employs a two-site sandwich ELISA to quantitate SCYE1 in samples. An antibody specific for SCYE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCYE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCYE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCYE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 microplate
• Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 standard
• Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (SCYE1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCYE1. No significant cross-reactivity or interference between Human SCYE1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: AIMP1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.EMAPII is identical to the p43 subunit invariably associated with 9 aminoacyl-tRNA synthetase complexes. They stated that the C-terminal domain of p43 is an RNA-binding domain. EMAPII may be a bifunctional protein, binding RNA as part of the tRNA synthetase complexes and, after proteolytic cleavage in tumor cells, stimulating inflammatory responses.

Alternative Names: SCYE1; EMAP2; EMAPII; SCYE1; p43; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide; multisynthetase complex auxiliary co

Search name: SCYE1; EMAP2; EMAPII; SCYE1; p43; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide; multisynthetase complex auxiliary co

Tag: SCYE1