Human Annexin A4 (ANX-A4) ELISA Kit | KTE62440

Human Annexin A4 (ANX-A4) ELISA Kit | KTE62440 | Gentaur UK, US & Europe Distribution

Application: This Human Annexin A4 (ANX-A4) ELISA Kit employs a two-site sandwich ELISA to quantitate ANXA4 in samples. An antibody specific for ANXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Annexin A4 microplate
• Human Annexin A4 standard
• Human Annexin A4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Annexin A4 (ANX-A4) ELISA Kit has high sensitivity and excellent specificity for detection of Human ANXA4. No significant cross-reactivity or interference between Human ANXA4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways.ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells.

Alternative Names: ANXA4; ANX4; DKFZp686H02120; MGC75105; PIG28; ZAP36; annexin IV; annexin IV (placental anticoagulant protein) ; placental anticoagulant protein; proliferation-inducing gene 28; proliferation-inducing prot

Search name: ANXA4; ANX4; DKFZp686H02120; MGC75105; PIG28; ZAP36; annexin IV; annexin IV (placental anticoagulant protein) ; placental anticoagulant protein; proliferation-inducing gene 28; proliferation-inducing prot

Tag: ANXA4