Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) ELISA Kit | ELK8071

Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) ELISA Kit | ELK8071 | Gentaur UK, US & Europe Distribution

Alternative Names: Anti-Spermatozoa Antibodies; Sperm Antibodies; Antisperm Antibodies

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 1.17 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h, 30min

Research Area: Reproductive science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in the samples is then determined by comparing the OD of the samples to the standard curve.