740
Human Anti-ryanodine receptor (RyR) calcium release channel antibody ELISA Kit | AE62571HU
- SKU:
- 740-AE62571HU
- Availability:
- Usually ships in 5 working days
Description
Human Anti-ryanodine receptor (RyR) calcium release channel antibody ELISA Kit | AE62571HU | Gentaur UK, US & Europe Distribution
Species Reactivity: Human (Homo sapiens)
Abbreviation: Anti-ryanodine receptor (RyR) calcium release channel antibody
Alternative Name: N/A
Application: ELISA
Range: 0.781-50 ng/mL
Sensitivity: 0.27 ng/mL
Intra-Assay: ≤3.7%
Inter-Assay: ≤7.2%
Recovery: 1, 04
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Quantitive
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-ryanodine receptor (RyR) calcium release channel antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated Anti-ryanodine receptor (RyR) calcium release channel antibody and incubated. The competitive inhibition reaction is launched between with HRP labeled Anti-ryanodine receptor (RyR) calcium release channel antibody and unlabeled Anti-ryanodine receptor (RyR) calcium release channel antibody with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Anti-ryanodine receptor (RyR) calcium release channel antibody in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: Ryanodine receptor 1 is a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described.Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.Skeletal muscle and brain (cerebellum and hippocampus) .
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .