Human Anti-synaptophysin antibody (SYP-Ab) ELISA Kit | KTE62470

Human Anti-synaptophysin antibody (SYP-Ab) ELISA Kit | KTE62470 | Gentaur UK, US & Europe Distribution

Application: This Human Anti-synaptophysin antibody (SYP-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SYP-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated SYP-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled SYP-Ab and unlabeled SYP-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SYP-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Anti-synaptophysin antibody microplate
• Human Anti-synaptophysin antibody standard
• Human Anti-synaptophysin antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Anti-synaptophysin antibody (SYP-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYP-Ab. No significant cross-reactivity or interference between Human SYP-Ab and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has lead to the use of synaptophysin immunostaining for quantification of synapses.The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally.Synaptophysin can refer both to the vesicle membrane protein.

Alternative Names: SYP-Ab

Search name: SYP-Ab

Tag: SYP