Human ANXA6 (Annexin A6) ELISA Kit | ELK3041

Human ANXA6 (Annexin A6) ELISA Kit | ELK3041 | Gentaur UK, US & Europe Distribution

Alternative Names: ANX6; ANX-A6; CBP68; CPB-II; Annexin VI; 67 kDa calelectrin; Calphobindin-II; Chromobindin-20; Lipocortin VI

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A6 (ANXA6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Annexin A6 (ANXA6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A6 (ANXA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A6 (ANXA6) in the samples is then determined by comparing the OD of the samples to the standard curve.