Human AP17 (Apelin 17) ELISA Kit | ELK4210

Human AP17 (Apelin 17) ELISA Kit | ELK4210 | Gentaur UK, US & Europe Distribution

Alternative Names: APLN17

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 9.14 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Central nervous system ；cardiovascular system

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin 17 (AP17) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin 17 (AP17) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin 17 (AP17) in the samples is then determined by comparing the OD of the samples to the standard curve.