Human APLNR (Apelin Receptor) ELISA Kit | ELK8782

Human APLNR (Apelin Receptor) ELISA Kit | ELK8782 | Gentaur UK, US & Europe Distribution

Alternative Names: AGTRL1; HG11; APJR; APJ; APJ Receptor; Angiotensin II Receptor-Like 1; Angiotensin receptor-like 1

Species: Human

Assay Type: Sandwich

Sensitivity: 6.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apelin Receptor (APLNR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin Receptor (APLNR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apelin Receptor (APLNR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin Receptor (APLNR) in the samples is then determined by comparing the OD of the samples to the standard curve.