Human Apolipoprotein E (APOE) ELISA Kit | AE59361HU

Human Apolipoprotein E (APOE) ELISA Kit | AE59361HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: APOE

Alternative Name: AD2; LDLCQ5; LPG; MGC1571; apolipoprotein E3

Application: ELISA

Range: 4.7-300 ng/mL

Sensitivity: 1.8 ng/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤7.9%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate APOE in samples. An antibody specific for APOE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPOE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Apolipoprotein E has many functions in the body. When it is synthesized by the liver as part of VLDL it functions in the transport of triglycerides to the liver tissue. It is also incorporated into HDL (as HDL-E) and functions in cholesterol distribution among cells. It is also incorporated into intestinally synthesized cholymicrons and transports dietary triglycerides and cholesterol. It is involved in lipid metabolism by mediating the receptor binding of apo-E lipoproteins to the LDL receptor. Receptor binding begins the cellular uptake of lipoproteins to be used in intracellular cholesterol metabolism.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .