Human ARPC2 (Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit | ELK4658

Human ARPC2 (Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit | ELK4658 | Gentaur UK, US & Europe Distribution

Alternative Names: ARC34; PNAS-139; p34-Arc; Arp2/3 complex 34 kDa subunit

Species: Human

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Related Protein 2/3 Complex Subunit 2 (ARPC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) in the samples is then determined by comparing the OD of the samples to the standard curve.