Human ATG7 (Autophagy Related Protein 7) ELISA Kit | ELK7079

Human ATG7 (Autophagy Related Protein 7) ELISA Kit | ELK7079 | Gentaur UK, US & Europe Distribution

Alternative Names: APG7L; GSA7; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Ubiquitin-activating enzyme E1-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.112 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7 (ATG7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Autophagy Related Protein 7 (ATG7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7 (ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 7 (ATG7) in the samples is then determined by comparing the OD of the samples to the standard curve.