Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit | ELK1394

Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit | ELK1394 | Gentaur UK, US & Europe Distribution

Alternative Names: Neurotrophin; Abrineurin

Species: Human

Assay Type: Sandwich

Sensitivity: 12.7 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the OD of the samples to the standard curve.