Human Beta-2-glycoprotein 1 (APOH) ELISA Kit | KTE62676

Human Beta-2-glycoprotein 1 (APOH) ELISA Kit | KTE62676 | Gentaur UK, US & Europe Distribution

Application: This Human Beta-2-glycoprotein 1 (APOH) ELISA Kit employs a two-site sandwich ELISA to quantitate APOH in samples. An antibody specific for APOH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPOH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Beta-2-glycoprotein 1 microplate
• Human Beta-2-glycoprotein 1 standard
• Human Beta-2-glycoprotein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Beta-2-glycoprotein 1 (APOH) ELISA Kit has high sensitivity and excellent specificity for detection of Human APOH. No significant cross-reactivity or interference between Human APOH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Apolipoprotein H (ApoH), also known as Beta 2-glycoprotein I, is a plasma glycoprotein either circulating as a free protein or associated to lipoproteins. This protein was found in human serum and described for the first time in 1961 by Schultze et al. ApoH is a 54-kDa single-chain glycoprotein consisting of five carbohydrate chains and 326 amino acid residues that can be divided into five short consensus repeat domains.ApoH has been chiefly focused on as the cofactor or autoantigen in antiphospholipid syndrome (APS), which is related to venous and arterial thrombosis, fetal loss, and thrombocytopenia. Reports show that ApoH may have an important function in blood coagulation and clearance of apoptotic bodies from the circulation.

Alternative Names: APOH; B2G1; BG; apolipoprotein H

Search name: APOH; B2G1; BG; apolipoprotein H

Tag: APOH