Human BLVRB (Biliverdin Reductase B) ELISA Kit | ELK4002

Human BLVRB (Biliverdin Reductase B) ELISA Kit | ELK4002 | Gentaur UK, US & Europe Distribution

Alternative Names: FLR; SDR43U1; Flavin Reductase, NADPH; Short Chain Dehydrogenase/Reductase Family 43U, Member 1; Biliverdin-IX beta-reductase; Green heme-binding protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase B (BLVRB) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biliverdin Reductase B (BLVRB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase B (BLVRB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biliverdin Reductase B (BLVRB) in the samples is then determined by comparing the OD of the samples to the standard curve.