Human BNIP3L (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like) ELISA Kit | ELK9461

Human BNIP3L (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like) ELISA Kit | ELK9461 | Gentaur UK, US & Europe Distribution

Alternative Names: BNIP3a; BNIP3H; BNIP3L; NIP3 like protein X; NIP3L; NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like

Species: Human

Assay Type: Sandwich

Sensitivity: 0.094 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) in the samples is then determined by comparing the OD of the samples to the standard curve.