Human CA19-9 (Carbohydrate Antigen 19-9) ELISA Kit | ELK1198

Human CA19-9 (Carbohydrate Antigen 19-9) ELISA Kit | ELK1198 | Gentaur UK, US & Europe Distribution

Alternative Names: sialylated Lewis (a) Antigen; Cancer Antigen 19-9

Species: Human

Assay Type: Sandwich

Sensitivity: 0.28 U/mL

Standard: 50 U/mL

Detection Range: 0.79-50 U/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Hepatology;Gastroenterology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbohydrate Antigen 19-9 (CA19-9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carbohydrate Antigen 19-9 (CA19-9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbohydrate Antigen 19-9 (CA19-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Antigen 19-9 (CA19-9) in the samples is then determined by comparing the OD of the samples to the standard curve.