Human Cartilage glycoprotein 39 (HC gp-39) ELISA Kit | KTE62652

Human Cartilage glycoprotein 39 (HC gp-39) ELISA Kit | KTE62652 | Gentaur UK, US & Europe Distribution

Application: This Human Cartilage glycoprotein 39 (HC gp-39) ELISA Kit employs a two-site sandwich ELISA to quantitate HC gp39 in samples. An antibody specific for HC gp39 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHC gp39 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HC gp39 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HC gp39 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cartilage glycoprotein 39 microplate
• Human Cartilage glycoprotein 39 standard
• Human Cartilage glycoprotein 39 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cartilage glycoprotein 39 (HC gp-39) ELISA Kit has high sensitivity and excellent specificity for detection of Human HC gp39. No significant cross-reactivity or interference between Human HC gp39 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Cartilage glycoprotein-39 is one of the major secreted proteins of ex vivo cultured human articular chondrocytes, synovial cells, and macrophages and also expressed by mature neutrophils (from myelocyte-metamyelocyte stage) . High levels of YKL-40 are found in synovial fluid from patients with active rheumatoid arthritis (RA) and in the hippocampus of schizophrenic patients. YKL-40 belongs to the 18-glycosyl hydrolase (mammalian chitinase) family, and is one of the putative autoantigens in rheumatoid arthritis (RA) . High levels of YKL-40 are found in synovial fluid from patients with active RA. The name 'YKL' stands for its N-terminal tripeptide sequence, and unlike the other member YKL39 which is unglycosylated, YKL40 is glycosylated.

Alternative Names: HC gp39

Search name: HC gp39

Tag: HC gp39